app saa mice  (Jackson Laboratory)

Journal: bioRxiv

Article Title: Therapeutic targeting of fibrin–microglia interactions ameliorates Alzheimer’s disease-related hyperexcitability and brain network dysfunction

doi: 10.64898/2026.05.01.722324

Figure Lengend Snippet: Brain tissue from human AD (A) and from 5xFAD, App SAA/SAA , App NLG/NLG , and App NLG/NLG :APOE4 mice (B-D) were stained for fibrin(ogen) (red), Aβ (blue), and the microglia marker Iba1 (green) primary antibodies (scale bar, 25 mm). ( A ) In AD brain tissue, fibrin(ogen) deposition was found within amyloid plaques (#), diffusely around some of the blood vessels (*), and decorating blood vessels (arrowheads). Representative images are shown from n = 3 samples. ( B ) 10-month-old 5xFAD mice showed robust fibrin(ogen) deposition within and around amyloid plaques and decorating blood vessels (arrowheads). Reactive microglia (green) accumulated around amyloid and fibrin(ogen) deposits. Representative images are shown from n = 10 samples. ( C, D ) Relative to 5xFAD mice (B), 21-month-old App NLG/NLG (C) and 15-month-old App NLG/NLG :APOE4 (D) mice showed more diffuse fibrin(ogen) deposition around amyloid plaques, with fibrin(ogen) forming an external halo around amyloid plaques. Reactive microglia cells (green) accumulated around amyloid and fibrin(ogen) deposits. Representative images are shown from n = 6–10 samples per genotype.

Article Snippet: App SAA mice were obtained from The Jackson Laboratory (Jax 034711).

Techniques: Staining, Marker

Journal: Scientific Reports

Article Title: Longitudinal characterization reveals behavioral impairments in aged APP knock in mouse models

doi: 10.1038/s41598-025-89051-8

Figure Lengend Snippet: APP-KI mice do not show progressive cognitive impairment through 12 months of age. Male and female APP SAA ( a , c , e , g ) and APP WT ( b , d , f , h ) mice were tested longitudinally from 4 through 12 month (m) of age, at the indicated time points across a battery of cognitive behavior tests. ( a , b ) Exploration ratio in the object location test (OLT). ( c , d) Percent alternation in the Y-maze. ( e , f ) Barnes maze performance during longitudinal training period and ( g , h ) 72 h probe trial. ( i , j ) Tris soluble and Triton soluble hippocampal Aß analyzed by multiplex ELISA. ( i ) Total Aβ including Aβ38, Aβ40, and Aβ42 ( j ) Aβ 42:40 ratio. A significant genotype by solubility interaction effect was observed in Aβ 42:40 ratio ( p = 0.0297). Points represent individuals (excluding ( e , f ) where points represent the group mean), bars represent the mean ± SEM. n = 10–11 APP WT and 13 APP SAA for ( a-h ) and n = 4 for ( i-j ). Data analyzed by one sample t test compared to 0.5 chance level for ( a , b ) repeated measures ANOVA and Dunnett’s multiple comparisons test to compare each age to 4 m timepoint ( c-h ) and 2-way ANOVA with Fisher’s LSD post-hoc tests I-J. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: Female and male APP SAA KI (B6.Cg-Apptm1.1Dnli/J Strain #:034711) and APP WT KI (B6.Cg-Appem1Adiuj/J Strain #:033013) mice were acquired from Jackson Labs through a kind gift by Dr. Srikant Rangaraju (Emory University) and maintained as homozygotes.

Techniques: Battery, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Solubility

Journal: Scientific Reports

Article Title: Longitudinal characterization reveals behavioral impairments in aged APP knock in mouse models

doi: 10.1038/s41598-025-89051-8

Figure Lengend Snippet: APP SAA but not APP WT mice show spatial learning and memory deficits at 16 months of age. Young (2–3-month-old) and 16-month-old male and female APP SAA and APP WT mice were tested cross sectionally on a range of cognitive tests. ( a ) Exploration ratio in the object location test (OLT). ( b ) Percent alternation in the Y-maze. ( c-e ) Barnes maze performance during longitudinal training period showing both ( c ) primary latency and ( d ) area under the curve (AUC). ( e ) 72 h probe trial primary latency. Points represent individuals (excluding c , where points represent the group mean), bars represent the mean and SEM. n = 9–12 APP WT and 9–13 APP SAA . Data analyzed by one sample t test compared to 0.5 chance level for ( a ), 2-way ANOVA with Fisher’s LSD post hoc test for ( b , d and e ). * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: Female and male APP SAA KI (B6.Cg-Apptm1.1Dnli/J Strain #:034711) and APP WT KI (B6.Cg-Appem1Adiuj/J Strain #:033013) mice were acquired from Jackson Labs through a kind gift by Dr. Srikant Rangaraju (Emory University) and maintained as homozygotes.

Techniques: